Serum concentration and vascular expression of adiponectin are differentially associated with the diabetic calcifying peripheral arteriopathy

Carole Aubert (1) , Sophie Liabeuf (2, 3) , Chloé Amouyal (4) , Salim Kemel (5, 1) , Frédérique Lajat-Kiss , Jean-Marc Lacorte (6) , Marine Halbron (1) , Aurélie Carlier (6) , Joe-Elie Salem (6) , Christian Funck-Brentano (6) , Ljubica Perisic Matic , Anna Witasp , Peter Stenvinkel (7) , Franck Phan (1, 6, 8) , Ziad Massy (9, 10, 11) , Agnès Hartemann (1) , Olivier Bourron (8, 12, 1)

1 CHU Pitié-Salpêtrière [AP-HP]
2 CHU Amiens-Picardie
3 MP3CV - Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517
4 Nutriomics - Nutrition et obésités: approches systémiques (UMR-S 1269)
5 LIB - Laboratoire d'Imagerie Biomédicale [Paris]
6 ICAN - Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases
7 Karolinska Institutet [Stockholm]
8 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers
9 Hôpital Ambroise Paré [AP-HP]
10 CESP - Centre de recherche en épidémiologie et santé des populations
11 UVSQ - Université de Versailles Saint-Quentin-en-Yvelines
12 IHU ICAN - Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière]

Background: Medial calcification in diabetes contributes to the arterial occlusive process occurring below the knee level. Adiponectin is an adipokine with atheroprotective properties and possible protective role against arterial calcification. The aim of the study was to investigate, in type 2 diabetes, the link between vascular expression and serum concentration of adiponectin and (1) peripheral arterial calcification and (2) lower limb occlusive arterial disease.Methods: Scoring of peripheral vascular calcification and peripheral arterial occlusive disease, using CT-scan and color-duplex ultrasonography respectively, were conducted and explored in relation to serum adiponectin level in a cross sectional study of 197 patients with type 2 diabetes. Vascular adiponectin expression in the arterial wall of diabetic patients with and without medial calcification was evaluated by immunohistochemistry.Results: Peripheral arterial calcification score was higher in patients with the highest adiponectin concentration. In a multivariate logistic regression analysis, an increase of 1 µg/mL of adiponectin was associated with a 22% increase of arterial calcification (adjusted OR = 1.22; 95% CI 1.03–1.44; p = 0.02). Arterial occlusive score was also higher in patients with adiponectin concentration > median (2.8 ± 4.8 vs 4.2 ± 5.7, p = 0.034). Immunohistochemical analyses showed a strong and specific staining of adiponectin in smooth muscle cells in calcified arteries, with a more pronounced expression of adiponectin in early stages of medial calcification.Conclusions: Peripheral arterial calcification is positively associated with circulating adiponectin levels in patients with type 2 diabetes, but vascular adiponectin expression is already observed at early stages of calcification. Adiponectin secretion could be a compensatory mechanism against the calcification process.

Mots clés: Vascular calcifcation; Adiponectin; Type 2 diabetes; Peripheral arterial disease

Domaines: Endocrinologie et métabolisme; Cardiologie et système cardiovasculaire

Carole Aubert, Sophie Liabeuf, Chloé Amouyal, Salim Kemel, Frédérique Lajat-Kiss, et al.. Serum concentration and vascular expression of adiponectin are differentially associated with the diabetic calcifying peripheral arteriopathy. Diabetology and Metabolic Syndrome, 2019, 11 (2), pp.32. ⟨10.1186/s13098-019-0429-7⟩. ⟨hal-02125115⟩